Fig. 2

Temporal expression pattern analysis of slc2a10 during development and in adult zebrafish tissues. RNA samples were purified from different embryonic and larval stages and from adult fishes and amplified with specific primer pairs of slc2a10. RT-PCR analysis (A) showed that two splice variants were expressed both maternally and at different developmental stages, similar to adult fish. Q-PCR (B) was used to quantify the expression levels of total slc2a10 mRNA during embryogenesis and in adult fishes. Expression of ef-1α housekeeping gene was used to normalize the relative expression levels of slc2a10 mRNA, which were compared to the corresponding values of the 1-cell stage (set to 1). Two specific PCR products of 727 and 550 bp were amplified from total RNA purified from adult zebrafish liver, heart, brain, eyes, gills, spleen, ovary, swim bladder, caudal fins, intestine, testes and kidney (C). To assess the RNA quality, a β-actin mRNA-specific product was obtained from all tissues analyzed. Abbreviations: A, adult; M, Size Marker.