FIGURE

Fig. 2

ID

ZDB-FIG-120316-17

Publication

Zannino et al., 2012 - Olig2-expressing hindbrain cells are required for migrating facial motor neurons

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Fig. 2

Facial motor neurons fail to completely exit r4 and r5 to form clusters in r6 and r7. Panels show a single z-plane image of dorsally mounted whole embryos with anterior to the left. Tg(isl1:GFP)^rw0 reporter expression, amplified using an anti-GFP antibody and an Alexa 488 secondary antibody, is revealed as green and RNA expression patterns are blue. A,B:hoxb1a expression marks r4. C–F:egr2b expression labels r3 and r5. G–L:hoxb4a expression begins at the r6/r7 boundary and extends posteriorly. Arrows indicate region where facial motor neurons normally migrate. Approximate location of the otic vesicles is outlined with red ovals. Scale bar = 32 μm.

Expression Data

Genes:	egr2b GFP hoxb1a hoxb4a
Fish:	rw0Tg rw0Tg + MO4-olig2
Knockdown Reagent:	MO4-olig2
Anatomical Terms:	facial nerve motor nucleus motor neuron rhombomere 3 rhombomere 4 rhombomere 5 rhombomere 7
Stage Range:	20-25 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg + MO4-olig2
Knockdown Reagent:	MO4-olig2
Observed In:	facial nerve motor nucleus motor neuron neuron migration
Stage Range:	20-25 somites to Prim-15

Phenotype Detail

Acknowledgments

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