FIGURE
Fig. S2
- ID
- ZDB-FIG-120315-55
- Publication
- Choorapoikayil et al., 2012 - Haploinsufficiency of tumor suppressor pten predisposes to hemangiosarcoma in zebrafish
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Fig. S2
PTEN antibody specifically recognizes Ptena and not Ptenb protein. Protein lysate from 3 dpf wild type, ptena-/- and ptenb-/- embryos were run on an SDS-polyacrylamide gel. The proteins were transferred to a PVDF membrane and after blocking the blot was probed with anti-PTEN antibody, stripped and sequentially probed with actin as a loading control. Detection was by enhanced chemiluminescence and representative blots are shown. Specific signal is detected in wild type and ptenb-/- embryos, but not in ptena-/- embryos, indicating that the PTEN antibody is specific for Ptena. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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