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Fig. S4

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ZDB-FIG-120216-83

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Merrick et al., 2012 - The γ-Secretase Cleavage Product of Polycystin-1 Regulates TCF and CHOP-Mediated Transcriptional Activation through a p300-Dependent Mechanism

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Fig. S4

Morpholino-mediated knockdown of Pkd1a/b results in dorsal axis curvature in zebrafish embryos, which is not altered by expression of the PC1-CTTΔNLS. (a) Morpholinos corresponding to the zebrafish Pkd1a and Pkd1b Polycystin-1 genes were injected into zebrafish embryos at the 1-2 cell stage to impair expression of the two Pkd1 genes. The embryos were subsequently injected with 300ng of mRNA encoding HA-PC1-CTTΔNLS, where indicated, and imaged at 3dpf. (b) Embryo phenotypes were scored based on the degree of dorsal tail curvature: mild <90 degrees, moderate >90 degrees and severe = tail tip crossing the body axis. The data represent the averages of four separate experiments; error bars represent standard error of the mean (s.e.m.).

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Cell, 22(1), Merrick, D., Chapin, H., Baggs, J.E., Yu, Z., Somlo, S., Sun, Z., Hogenesch, J.B., and Caplan, M.J., The γ-Secretase Cleavage Product of Polycystin-1 Regulates TCF and CHOP-Mediated Transcriptional Activation through a p300-Dependent Mechanism, 197-210, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell