Fig. S1
- ID
- ZDB-FIG-120202-41
- Publication
- Kuo et al., 2011 - Soluble THSD7A Is an N-Glycoprotein That Promotes Endothelial Cell Migration and Tube Formation in Angiogenesis
- Other Figures
- All Figure Page
- Back to All Figure Page
The release of soluble THSD7A is protease-dependent. A. THSD7A-transfected HEK293T cells were treated with complete protease inhibitor (C), Benzamidine (B), Leupeptin (L) and Pepstatin A (P). Untreated cells (No) served as a control. Cell lysates and cultured medium were subjected to Western blot with anti-sTHSD7A antibody. GAPDH served as a loading control. B. Two proteases, thrombin and caspase 1, are predicted to cleave the full-length THSD7A. C. THSD7A-transfected HEK293T cells were treated with complete protease inhibitor (C), hirudin (H), caspase 1 inhibitor I (I) or no treatment (No). Cell lysates and cultured medium were subjected to Western blot with anti-sTHSD7A antibody. GAPDH served as a loading control. |