Fig. S7
- ID
- ZDB-FIG-120201-33
- Publication
- Kagermeier-Schenk et al., 2011 - Waif1/5T4 Inhibits Wnt/β-Catenin Signaling and Activates Noncanonical Wnt Pathways by Modifying LRP6 Subcellular Localization
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Waif1a is a positive regulator of Wnt/PCP signaling, related to figure 7. (A-C) waif1a enhances wnt11 signaling. (A-B) Zebrafish embryos injected with 200 pg wnt11 RNA plus 60 pg RLuc or equimolar amounts (75 pg) of waif1a RNA photographed at 24 hpf. (C) wnt11 induced phenotypes were classified into 4 categories (not shown). Class I have hindbrain convergence defects, class 2 in addition cyclopia, class 3 the above plus shortened trunk/tail and class 4 lack recognizable trunk / tail structures. The phenotypes were counted in embryos injected with moderate (100 pg, left) or high (200 pg, right) doses of wnt11 RNA with or without 75 pg waif1a or 80 pg RLuc. waif1a RNA significantly enhanced moderate (*, p<0.05, Chi-Square test) and high doses (***, p<0.001) of wnt11. (D) waif1a genetically interacts with wnt5b. Images of the tail region of live zebrafish embryos at 24 hpf are shown. Embryos injected with 6 ng of waif1a MO1 plus 0.8 ng standard control MO have normal notochords (37/40 embryos). Likewise, low doses (0.8 ng) of wnt5b MO plus 6 ng control MO do not cause notochord defects (71/73). However, combined knockdown (6 ng waif1a MO1 plus 0.8 ng wnt5b MO) results in wavy notochords in 35/66 embryos. |
Reprinted from Developmental Cell, 21(6), Kagermeier-Schenk, B., Wehner, D., Ozhan-Kizil, G., Yamamoto, H., Li, J., Kirchner, K., Hoffmann, C., Stern, P., Kikuchi, A., Schambony, A., and Weidinger, G., Waif1/5T4 Inhibits Wnt/β-Catenin Signaling and Activates Noncanonical Wnt Pathways by Modifying LRP6 Subcellular Localization, 1129-43, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell