FIGURE

Fig. 7

ID

ZDB-FIG-120131-49

Publication

Du et al., 2012 - Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A

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Fig. 7

Cell morphology is altered in MZeomesa embryos. (A-D) Confocal images of embryos at sphere stage stained for Cdh1 (A,B) and β-Catenin (C,D). (E) Western blot of wild type (lane 1) and MZeomesa mutant embryo (lane 2) sphere stage extracts. Levels of Cdh1 and β-Catenin are not obviously altered in mutant embryos. (F) Normal cdh1 expression in sphere stage wild type, MZeomesa, eomesa-eng and eomesa-VP16 injected wild type embryos, as indicated. (G) Live DIC images of wild type and MZeomesa embryos at sphere stage. Mutant cells are more tightly packed and exhibit more blebs then wild type cells. Orange highlights selected cell morphologies, purple highlights intercellular space.

Expression Data

Gene:	cdh1
Antibody:	AB1-cdh1
Fish:	AB eomesa^fh105/fh105 (AB)
Anatomical Terms:	blastodisc whole organism
Stage:	Sphere

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eomesa^fh105/fh105 (AB)
Observed In:	blastodisc deep blastomere
Stage:	Sphere

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 362(1), Du, S., Draper, B.W., Mione, M., Moens, C.B., and Bruce, A.E., Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A, 11-23, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.