FIGURE

Fig. 3

ID

ZDB-FIG-111220-5

Publication

Doll et al., 2011 - Subnuclear development of the zebrafish habenular nuclei requires ER translocon function

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Fig. 3

The enlarged LsDh phenotype of sec61al1^c163 is independent of the parapineal organ. (A) In wild-type larvae, the parapineal organ (gfi-1, blue) is found adjacent to the left LsDh (kctd12.1, red). (A′) Likewise, sec61al1^c163 mutants possess a single parapineal organ on the left side of the brain. (B) The pineal complex (consisting of the pineal and parapineal, labeled by otx5) is similar in WT and (B′) sec61al1^c163 mutant larvae. (C) Compared to an non-injected control (NIC), with a parapineal organ on the left side of the brain (dashed oval), (D) disruption of parapineal organ formation using a tbx2b antisense morpholino causes a reduction in the size of the left LsDh (kctd12.1). (C′, D′) However, in sec61al1^c163 mutants, reduction of the parapineal organ has no effect on the size of the LsDh on either the left or right side. All views are dorsal. Panels A, C, D = 96 hpf; B = 72 hpf. Parapineal indicated by black arrowheads A–C′. Scale bar = 10 μm.

Expression Data

Genes:	gfi1ab kctd12.1 otx5
Fish:	AB sec61a1a^c163/c163 (AB) AB + MO1-tbx2b sec61a1a^c163/c163 + MO1-tbx2b (AB)
Knockdown Reagent:	MO1-tbx2b
Anatomical Terms:	dorsal habenular nucleus lateral region epiphysis parapineal organ
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-tbx2b sec61a1a^c163/c163 + MO1-tbx2b (AB)
Knockdown Reagent:	MO1-tbx2b
Observed In:	dorsal habenular nucleus lateral region dorsal habenular nucleus right side neuron parapineal organ
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 360(1), Doll, C.A., Burkart, J.T., Hope, K.D., Halpern, M.E., and Gamse, J.T., Subnuclear development of the zebrafish habenular nuclei requires ER translocon function, 44-57, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.