FIGURE

Fig. 4

ID

ZDB-FIG-111220-14

Publication

Kimmel et al., 2011 - Requirement for Pdx1 in specification of latent endocrine progenitors in zebrafish

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Fig. 4

Anterior NeuroD:EGFP cells are migratory endocrine precursors. (A-D) Confocal sections of 3 days post fertilization (dpf) TgBAC(NeuroD:EGFP)nl1; Tg(ins:dsRed)m1018 embryos colabeled with antibodies against green fluorescent protein (GFP) and duct marker 2F11 (in blue). GFP+/2F11+ cells emerge from the duct (arrows), some with long projections typical of migrating cells (A, B, arrowhead). An individual cell that has separated from the duct is weakly DsRed positive (C, arrowhead). (E) Confocal image projections from a timelapse sequence of a 3 dpf TgBAC(NeuroD:EGFP)nl1;Tg(-8.0cldnb:lynEGFP)zf106 double transgenic embryo [see Additional file [8]. Membrane-linked enhanced green fluorescent protein (EGFP) in Tg(-8.0cldnb:lynEGFP)zf106 embryos delineates the gut epithelium (blue outline = extrapancreatic duct (EPD)) while robust cytoplasmic EGFP is present in NeuroD-expressing cells. Two NeuroD:EGFP cells (arrow) adjacent to the EPD migrate to the principal islet. All embryos are shown from ventral view, with the anterior to the left. Scale bar = 15 μM.

Expression Data

Genes:	DsRed EGFP
Antibody:	Ab-2F11
Fish:	m1018Tg; nl1Tg nl1Tg; zf106Tg
Anatomical Terms:	endocrine pancreas endocrine pancreas endoderm extrapancreatic duct pancreatic B cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.