FIGURE

Fig. 3

ID

ZDB-FIG-111215-10

Publication

Oehlers et al., 2011 - The inflammatory bowel disease (IBD) susceptibility genes NOD1 and NOD2 have conserved anti-bacterial roles in zebrafish

Other Figures

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Fig. 3

Leukocytic response to yolk sac infection in control and morphant embryos. (A) Time course of Tg(lyzC:dsRed)⁵⁰ control and morphant embryos infected by yolk sac injection with S. enterica at 2 dpf. In control row: red arrows indicate embryos with a normal number of neutrophils in the CHT; blue arrows indicate embryos with a decreased number of neutrophils in the CHT. Scale bar: 1 mm. (B) Analysis of neutrophil number in the CHT of S. enterica-infected Tg(lyzC:dsRed)⁵⁰ embryos. Data represents three biological replicates; 50 embryos of each treatment were infected and followed for analysis. (C) Expression of l-plastin analyzed by qPCR. (D) Expression of gcsfr analyzed by qPCR. Data for qPCR experiments represents triplicate pools of five to ten embryos. Error bars represent s.e.m.; statistically significant differences of P<0.01 (**) as determined by ANOVA are marked.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nz50Tg nz50Tg + MO1-nod1 nz50Tg + MO1-nod2
Condition:	bacterial treatment by injection: Salmonella enterica subsp. enterica serovar Typhimurium
Knockdown Reagents:	MO1-nod1 MO1-nod2
Observed In:	blood island neutrophil
Stage Range:	Day 4 to Day 5

Phenotype Detail

Acknowledgments

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