Fig. 2

hi3959A is a zygotic null allele of qilin.

(A) Graphic representation of qilin gene. Blue squares represent exons. Red triangle indicates the proviral insertion site. Arrows indicate the direction and location of the genotyping primers used. (B) qilin transcript in embryos at 5 dpf shown by RT-PCR of cDNA from hi3959A embryos (lane 2) and wild type siblings (lane 1). Lane 3 and 4 are beta-actin loading controls for hi3959A (Lane 4) and wild type siblings (Lane 3). (C) Genotyping PCR of embryos from hi3959A carrier in-crosses injected with eGFP-qilin mRNA. All embryos genotyped exhibited wild type phenotypes. Lower band is specific for the mutant allele, while the upper band is specific for the wild type allele. From left to right, the three embryos are heterozygous, homozygous mutant and homozygous wild type, respectively. (D) Graph displaying percent embryos with the curved body phenotype in hi3959A carrier-in crosses that are uninjected (n = 3, with an average of 140 embryos per experiment) and injected with eGFP-qilin mRNA (n = 3, with an average of 120 embryos per experiment). * p< 0.05.