FIGURE

Fig. 9

ID

ZDB-FIG-111201-51

Publication

Mo et al., 2011 - Both pre- and postsynaptic activity of nsf prevents degeneration of hair-cell synapses

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Fig. 9

Partial rescue of synaptic contacts in nsf^st53 mutants by BDNF injection at 2dpf.

A, Representative z-projections of single neuromasts (4 dpf) immunolabeled for Zn12 (green) and Ribeye b (red) in wild-type, nsf^st53 mutants, and nsf^st53mutants injected with 5 μg/ml BDNF at 1dpf and 2dpf. 10 optical sections, 1 μm each. Scale bar: 10 μm. B, Quantification of total pixel intensity (A.U.) of Zn12 labeling in wild-type (1.478e6±128180, n = 10), nsf^st53 mutants (382343±26874, n = 15), and mutants with BDNF injections at 1dpf (513236±87981, n = 7), 2dpf (568136±88664, n = 6), 3dpf (339253±25527, n = 11). Statistical tests were between uninjected control and BDNF injected mutants.

Expression Data

Antibodies:	Ab3-ctbp2l zn-12
Fish:	WT nsfa^st53/st53
Anatomical Terms:	neuromast neuromast hair cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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