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Fig. 6

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ZDB-FIG-111103-11

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Asaduzzaman et al., 2011 - Multiple cis-elements in the 5'-flanking region of embryonic/larval fast-type of the myosin heavy chain gene of torafugu, MYH(M743-2), function in the transcriptional regulation of its expression

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Fig. 6

Effects of SRF-like binding sites deletions on EGFP expression in the myotomal compartments. A: Schematic representation showing SRF-like binding sites deleted one by one and the percentages of EGFP-expressing embryos (high, medium and low) in each SRF-deletion constructs. The total number of embryos injected with each construct is shown in parentheses. B-D: Lateral view showing high (B), medium (C) and low (D) EGFP expression in myotomal compartments in embryos injected with the P2075ΔSRF1-3 construct. E: Bar graph showing reduced relative EGFP expression in the P2075ΔSRF1-2 and P2075ΔSRF1-3 constructs using real-time PCR analysis. Differences are significant in ANOVA followed by Tukey test at *** P < 0.001.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Gene, 489(1), Asaduzzaman, M., Kinoshita, S., Siddique, B.S., Asakawa, S., and Watabe, S., Multiple cis-elements in the 5'-flanking region of embryonic/larval fast-type of the myosin heavy chain gene of torafugu, MYH(M743-2), function in the transcriptional regulation of its expression, 41-54, Copyright (2011) with permission from Elsevier. Full text @ Gene