FIGURE

Fig. 4

ID

ZDB-FIG-111102-4

Publication

Gutnick et al., 2011 - The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary

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Fig. 4

Morphogenesis of the Neurohypophysis

(A–C) Dynamics of hypophyseal innervation. Images of transgenic otpb:caax-EGFP embryos reveal that innervation of the hypophysis by neurons of the neurosecretory preoptic nucleus (NPO) occurs as early as 36 hr postfertilization and becomes well established by 54 hr. Scale bar, 20 μm.

(D–F) Dynamics of neurovascular interface. Images of double transgenic otpb:caax-EGFP;fli1:dsRed embryos at different developmental stages showing innervation of the neurohypophysis prior to hypophyseal vessel formation.

(G) Dynamics of hypophyseal vascularization. Time-lapsed (48–72 hr) confocal microscopy was used to track vascular endothelial cells in the ventral diencephalon of vegfr2:Cherry embryos. The upper panels display snapshots of critical stages in the formation of the hypophyseal loop-like structure. A schematic representation of the corresponding images with arterial (red) and venous (blue) color-coding is shown at the bottom.

CrDI, cranial division of the internal carotid artery; HyA, hypophyseal artery; HyV, hypophyseal veins; OA, optic artery; PHS, primary head sinus; PLA, palatocerebral artery. The asterisk (*) demarcates the location of the neurohypophysis. See also Movie S6.

Expression Data

Genes:	DsRedx EGFP mCherry
Fish:	s896Tg zc49Tg um13Tg; zc49Tg
Anatomical Terms:	hypophysis vascular endothelium neurohypophysis axon terminus neurosecretory neuron axon neurosecretory neuron axon terminus neurosecretory neuron neuronal cell body preoptic area neurosecretory neuron
Stage Range:	Prim-25 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 21(4), Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G., The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary, 642-654, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell