Fig. 5
The bistratified periventricular interneuron cell type. (A) Merged confocal image volume of a single 4 dpf bsPVIN expressing both dsRed (red) and Syp–GFP (green). (B) 2× magnification of bracketed region in (A). (C) Syp–GFP fluorescence of region in (D). Note the bright Syp–GFP puncta located within the arbor that targets the deeper synaptic layer (arrows in D), whereas puncta are absent from the more superficial arbor (arrowheads in C). (D) Schematic depiction of bsPVIN cell body distribution throughout the SPV layer. Cell traced in red indicates the relative location and scale of neuron depicted in (C). Scale bar, 20 μm in (A), 10 μm in (B,C). |