FIGURE

Fig. 7

ID

ZDB-FIG-110324-4

Publication

Shang et al., 2011 - Molecular analysis and functions of p53R2 in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Subcellular localization of zebrafish p53R2. ZF4 cells were transfected with plasmids expressing p53R2-Flag, GFP or p53R2-GFP. 24 h after transfection, cells were treated for 24 h with (CPT+) or without (CPT+) 100 nM CPT and then the following experiments were performed. (A) Real-time PCR analysis of endogenous p53R2 expression. (B) Immunofluorescence staining. Localization of p53R2 was detected with a primary anti-Flag antibody and a FITC-conjugated secondary antibody. Cell nuclei were stained with DAPI. (C) Localization of p53R2-GFP fusion protein in transfected cells was directly visualized under the green channel. Data are expressed as means ± SD from three independent experiments. * indicated p < 0.05 versus the control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene, 475(1), Shang, H., Li, Q., Feng, G., and Cui, Z., Molecular analysis and functions of p53R2 in zebrafish, 30-38, Copyright (2011) with permission from Elsevier. Full text @ Gene