FIGURE

Fig. 4

ID
ZDB-FIG-110322-57
Publication
van der Velden et al., 2011 - The serine-threonine kinase LKB1 is essential for survival under energetic stress in zebrafish
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Fig. 4

The zebrafish TOR (zTOR) pathway activity in lkb1 mutants resembles that in starved WT larvae. (A) Western blot analysis with antibodies against phospho-AMPK and phospho-ACC of total protein lysates from embryos at the indicated days and genotypes. AMPK and ACC are not phosphorylated in WT or lkb1-mutant larvae at 5 or 7 dpf. Prolonged fasting induces phosphorylation of AMPK and ACC in 11-dpf WT larvae. Elevated expression of AMPK in 7-dpf lkb1-mutant larvae is detected. (B) Western blot analysis using anti–phospho-p70S6K, anti-p70S6K, anti–phospho-4EBP1, anti-4EBP1, anti–phospho-RS6, and anti-RS6 antibodies of total protein lysates from embryos at the indicated days and genotypes. (C) Sections of livers obtained from WT and lkb1-mutant embryos at the indicated days stained with anti–phospho-RS6 and anti–phospho-4EBP1.
Expression Data
Antibodies: (all 10)
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Days 7-13

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Days 7-13

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA
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