FIGURE

Fig. 2

ID

ZDB-FIG-110317-12

Publication

Osborn et al., 2011 - Cdkn1c drives muscle differentiation through a positive feedback loop with Myod

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Myod-driven myogenin expression permits slow fibre formation in shha mutant. Myog mRNA or MyHC accumulation in uninjected, myod MO- or myog MO-injected embryos from wild type or shha^tbx/+ in-cross. Dorsal flatmounts of 5 ss (A,B) or 10–15 ss (C) embryos. Bar: 100 &MU;m. (A). Myog mRNA is ablated by myod MO injection into wild type. (B) Myod and myog MOs each delay adaxial myogenesis. (C) Myod and myog, but not myf5, MOs ablate adaxial myogenesis in shha^tbx392 mutants (21/79), but not from siblings.

Expression Data

Genes:	en2a myf6 myod1 myog prdm1a
Antibodies:	Ab-A4.1025 Ab-F59
Fish:	WT shha^{tbx392/tbx392} WT + MO1-myod1 WT + MO1-myog WT + MO3-myf5 shha^{tbx392/tbx392} + MO1-myod1 shha^{tbx392/tbx392} + MO1-myog shha^{tbx392/tbx392} + MO3-myf5
Knockdown Reagents:	MO1-myod1 MO1-myog MO3-myf5
Anatomical Terms:	adaxial cell somite
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	shha^{tbx392/tbx392} WT + MO1-myod1 WT + MO1-myog shha^{tbx392/tbx392} + MO1-myod1 shha^{tbx392/tbx392} + MO1-myog shha^{tbx392/tbx392} + MO3-myf5
Knockdown Reagents:	MO1-myod1 MO1-myog MO3-myf5
Observed In:	skeletal muscle tissue development
Stage Range:	5-9 somites to 10-13 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.