FIGURE

Fig. 6

ID

ZDB-FIG-110214-39

Publication

Ravanelli et al., 2011 - The Actin Nucleator Cordon-bleu is Required for Development of Motile Cilia in Zebrafish

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Fig. 6

Reduction of Cobl causes a reduction of apical F-actin in Kupffer′s vesicle.

(A–B) Maximum intensity projection of 0.8 μm confocal z-stacks of Kupffer′s vesicle, stained for cilia and actin. cobl morphant embryos with short cilia show deficits in the amount of F-actin staining by phalloidin. (C) Bar graph of pixel intensity of F-actin in Kupffer′s vesicle in controls vs. cobl morphants. Morphant embryos exhibit a significant decrease in the amount of F-actin. (D–E) Confocal images of cells in Kupffer′s vesicle showing cilia and F-actin. (F) Bar graph of pixel intensity of apical F-actin in Kupffer′s vesicle cells in controls vs. cobl morphants. Morphant embryos exhibit a significant decrease in the amount of F-actin present at the apical surface of cells. Dashed lines outline Kupffer′s vesicle. Arrows demark cortical F-actin. F-actin (phalloidin), cilia (anti-acetylated tubulin), DNA (DAPI). n values: control = 12, MOa = 7. p values: C = 0.0012*, F = 0.0283*. Error bars represent one standard error from the mean. Scale bar represents 10 μm.

Expression Data

Antibody:	Ab1-tuba
Fish:	EKW EKW + MO1-cobl
Knockdown Reagent:	MO1-cobl
Anatomical Term:	Kupffer's vesicle motile cilium
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO1-cobl
Knockdown Reagent:	MO1-cobl
Observed In:	ciliated epithelial cell apical cortex ciliated epithelial cell filamentous actin Kupffer's vesicle ciliated epithelial cell
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 350(1), Ravanelli, A.M., and Klingensmith, J., The Actin Nucleator Cordon-bleu is Required for Development of Motile Cilia in Zebrafish, 101-111, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.