FIGURE

Fig. 4

ID

ZDB-FIG-101129-6

Publication

Chi et al., 2010 - Cardiac conduction is required to preserve cardiac chamber morphology

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Fig. 4

dco Cardiac phenotype is rescued by RNA injection of Cx46 orthologs as well as by myocardial specific expression of zebrafish cx46. (A and B) Myocardial specific expression of WT [Tg(cmlc2:cx46-GFP^s882)] and mutant [Tg(cmlc2:cx46mt-GFP^s925)] zebrafish Cx46-GFP fusion proteins lead to cell membrane enrichment. Outer curvature (OC), inner curvature (IC), and atrioventricular canal (AV) are outlined by dashed lines. Atr, atrium; Ven, ventricle. (C) Injection of WT zebrafish (WT zfcx46) or mouse Cx46 (mouseCx46) mRNA as well as myocardial specific expression of WT zebrafish Cx46-GFP fusion proteins [Tg(cmlc2:cx46-GFP^s882)] rescued the dco cardiac phenotype. On the other hand, injection of zebrafish cx46^s226 (MT zfcx46) or cx43 (zfcx43) mRNA as well as myocardial specific expression of zebrafish Cx46^s226-GFP [Tg(cmlc2:cx46mt-GFP^s925)] failed to rescue the dco cardiac phenotype. (D) However, injection of zebrafish cx46^s226 mRNA or myocardial specific expression of zebrafish Cx46^s226-GFP [Tg(cmlc2:cx46mt-GFP^s925)] in WT embryos resulted in dco cardiac phenotypes. In addition, RNA overexpression of WT murine Cx46 caused a dco cardiac phenotype in a small percentage of WT embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA