Fig. S4
- ID
- ZDB-FIG-101123-34
- Publication
- Knopf et al., 2010 - Dually inducible TetON systems for tissue-specific conditional gene expression in zebrafish
- Other Figures
- All Figure Page
- Back to All Figure Page
Drug dose dependency of Axin1-YFP induction in myl7:TetA-GBD Cherry; TetRE:Axin1-YFP double transgenic embryos. (A) Dose–response to indicated doses of drugs applied at 48 h postfertilization (hpf). YFP RNA was detected by whole-mount in situ hybridization 4 h postinduction, EtOH control-treated embryos are shown at 24 h postinduction. (B) Classes of YFP expression in heart used for quantification. (C) Quantification of data shown in A. **P = 0.002 (Fisher exact test), ***P < 0.001 (χ2 test), *P = 0.034 (χ2 test). (D) Dex is limiting for induction in myl7:TetA-GBD Cherry; TetRE:Axin1-YFP double transgenic embryos. The indicated doses of drugs were applied at 48 hpf, YFP RNA was detected by whole-mount in situ hybridization 24 h postinduction. (E) Quantification of data shown in D). ***P < 0.001 (χ2 test). (F) Dex is limiting for induction in TetRE:Axin1-YFP embryos injected with 90 pg TetA-GBD RNA. Embryos were treated with indicated doses of drugs at 7 hpf for 4.5 h. Axin1-YFP expression was detected by in situ hybridization and classified as in Fig S1. ***P < 0.001, *P = 0.019 (χ2 test). |