FIGURE

Fig. 3

ID

ZDB-FIG-101122-53

Publication

Ragvin et al., 2010 - Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3

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Fig. 3

Pancreatic buds of 48-hpf zebrafish embryos showing a reduction in the number of insulin-expressing cells and an increase of ghrelin-expressing cells in irx3a morphants. In situ hybridization with insulin (red) and ghrelin (blue) probes on control (A) and irx3a morphant embryos (B). Statistical support for the observation is given in Table 3. (C and D) irx3a morphants display a reduction in pancreatic α-cells. In situ hybridization with glucagon (red in C and D), somatostatin (red in E and F), and ghrelin (blue in C–F) probes on control (C and E) and irx3a morphant embryos (D and F). showing reduction in the number of α-cells (D) and increase of ghrelin-expressing cells (D and F) in the pancreatic bud of 48-hpf irx3a morphant embryos. The number of δ-cells is unaffected. Embryos are in ventral view. Statistical support for the observation is given in Table 4.

Expression Data

Genes:	gcga ghrl ins sst2
Fish:	WT WT + MO1-irx3a + MO2-irx3a
Knockdown Reagents:	MO1-irx3a MO2-irx3a
Anatomical Terms:	pancreatic bud ghrelin secreting cell pancreatic bud insulin secreting cell pancreatic bud pancreatic A cell pancreatic bud somatostatin secreting cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-irx3a + MO2-irx3a
Knockdown Reagents:	MO1-irx3a MO2-irx3a
Observed In:	D cell pancreatic A cell pancreatic B cell pancreatic bud pancreatic epsilon cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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