FIGURE

Fig. 2

ID

ZDB-FIG-101122-13

Publication

Appelbaum et al., 2009 - Sleep-wake regulation and hypocretin-melatonin interaction in zebrafish

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Fig. 2

A stable hcrt:EGFP transgenic line reveals the entire HCRT circuit of a living larva. (A–C) Two-photon imaging of a 7dpf stable transgenic larva with head to the left, dorsal views. (A and close-up B) Composite picture showing the HCRT cell bodies in the diencephalon (Di) and their processes in the hindbrain (HB) and spinal cord (SC) and in the telencephalon (Tel) directed toward the olfactory bulbs (OB). In A and B, white areas on both sides of the larva correspond to skin autofluorescence. Commissural projections are observed ventrally to the HCRT cell body clusters (C, arrow) and in the anterior telencephalon (B, arrow, commissura anterior). (D) Mosaic expression of a nonintegrated hcrt:EGFP transgene allowing the observation of a single HCRT neuron harboring both commissural (arrow) and ipsilateral processes.

Expression Data

Gene:	EGFP
Fish:	r18Tg
Anatomical Terms:	diencephalon cell body hindbrain neuron projection spinal cord neuron projection telencephalon neuron projection
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA