FIGURE

Fig. S1

ID

ZDB-FIG-101108-30

Publication

Jänicke et al., 2010 - Zebrafish grainyhead-like1 is a common marker of different non-keratinocyte epidermal cell lineages, which segregate from each other in a Foxi3-dependent manner

Other Figures

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Fig. S1

Efficacy controls of used ATG- and splice grhl1 morpholinos. (A-C) Live embryos at late blastula stages that were injected with constructs indicated at the top for genes indicated at the left side. These images show that the three ATG-MOs targeted specifically and efficiently the sequence they were designed against. (D) Graphical illustration of the grhl1-splice MO targeting site and the primer binding sites used to control the efficacy. (E) The grhl1-splice MO interfered with splicing of intron3-4, indicated by the RT-PCR amplification of an exon3-intron3/4 fragment from morphant (MO), but not from uninjected control WT embryos (WT). In reverse, a combination of an exon3 and an exon4 primer only gave an RT-PCR amplification product from WT, but not from morphant embryos (data not shown). Intron3/4 contains an in frame stop codon after the third exon (highlighted in red in D), which will yield a C-terminally truncated Grhl1 protein.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.