Fig. 6

Differentiation and migration of olig2⁺ eurydendroid cells. (A–F) Expression of olig2 (B, D, F) and olig2:EGFP (A, C, E) in the cerebellum at 2, 3, and 5 dpf. Dorsal views with rostral to the left. (G–J) Expression of olig2:EGFP and vglut2:DsRed at 3 (G, H) and 5 (I, J) dpf. EGFP and DsRed signals in Tg(olig2:EGFP); Tg(vglut2a:DsRed) were detected with anti-EGFP (green) and anti-DsRed (magenta) antibodies, respectively. (H, J) Sagittal sections at the levels indicated in G and I, respectively. The olig2:EGFP⁺ cells in the VZ were negative for vglut2a:DsRed (arrowheads), but in the GCL and PCL domains, most olig2:EGFP⁺ cells were positive for vglut2a:DsRed (arrows). (K–O) Expression of olig2:EGFP and ptf1a:Gal4VP16; UAS:NfsB-mCherry (ptf1a:G4-U:mCherry) at 2 dpf (K, L), 5 dpf (M, N), and the adult cerebellum (O). (K, M) Dorsal views; (L) high-magnification view of the box in (K); (N) sagittal section at the level indicated in (M); (O) sagittal section. Note that most olig2:EGFP⁺ cells were also positive for ptf1a:G4-UAS:mCherry at 2 dpf (arrows), but many of them became negative after differentiation at 5 dpf. olig2:EGFP⁺ ptf1a:G4-U:mCherry⁺ cells were also detected in the VZ at 5 dpf and adult stage (arrowheads). (P-S) Expression of olig2:EGFP and atoh1a:dTomato at 2 (P, Q) and 5 (R, S) dpf. (Q, S) High-magnification views of the boxes in (P, R). The olig2:EGFP⁺ cells located in the lateral domains were positive for atoh1a:dTomato (arrows). (T) Schematic representation of development of the olig2⁺ eurydendroid cells. The abbreviations are defined in Fig. 1. Scale bars: 50 μm (H, S), 100 μm (L), 200 μm (A, B, G, K, O, P).