FIGURE

Fig. 12

ID

ZDB-FIG-100616-103

Publication

Insinna et al., 2010 - Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development

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Fig. 12

Double dnct1a/b knock-down primarily affects the inner segment. (A) TEM view of the central retina of a dctn1a/b morphant at 3 days showing normal, albeit short, outer segment structures. (B-F) TEM views of individual photoreceptors at the peripheral dctn1a/b morphant retina with inner segment polarization defects, including a dispersion of mitochondria (M) within that region (B, C, F), lack of a distinct myoid region (D) and positioning of the ER/Golgi complex among dispersed mitochondria (arrows in (B, E, F)). In addition, accumulation of Golgi associated vesicles in the inner segment (arrow in (B, E, F)) and shortened outer segment structures (arrowheads) were also seen (E, F). (G-I) Higher magnification insets of the red boxed regions. For morpholino (MO) injections, dnct1a splice MO was used at 2.5 ng/embryo, while the dnc1b splice MO was used at 8.4 ng/embryo (for a total MO concentration of 10.9 ng/embryo). BB, basal body; C, centriole; CC, connecting cilium; ER; endoplasmic reticulum; IS, inner segment; M, mitochondria; OS, outer segment. Scale bar: 2 μm in (A); 0.5 μm in (B-F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-dctn1a + MO2-dctn1b
Knockdown Reagents:	MO2-dctn1a MO2-dctn1b
Observed In:	eye photoreceptor cell Golgi apparatus eye photoreceptor cell photoreceptor inner segment eye photoreceptor cell photoreceptor outer segment photoreceptor cell morphogenesis
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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