Fig. S3

Selective PPARγ drug responses (a) At 24 hpf PPARγ embryos were subjected to a 30 minute heat shock at 37°C and then incubated for 24 hr in the presence of agonists specific for one of the three PPAR isoforms (Rosiglitazone for γ, GW0742 for δ/β and GW9578 or GW590735 for α). The concentrations chosen represent known EC50 values for the appropriate targets, along with significantly higher levels to test for cross-reactivity. Lateral views of 48 hpf embryos, anterior to the left, are shown. (b) PPARγ agonist/antagonist replacement. At 24 hpf PPARγ embryos were subjected to a 30 minute heat induction at 37°C and either incubated for 24 hr in the presence of solvent or 1000 nM GW9662 (upper row) or 50 nM Rosiglitazone (RGZ) alone or 50 nM RGZ and increasing concentrations of GW9662 (20 nM and 500 nM). Two embryos for each treatment showing lateral views at 48 hpf, anterior to the left, are shown.