FIGURE

Fig. 5

ID

ZDB-FIG-100323-7

Publication

Chablais et al., 2010 - IGF signaling between blastema and wound epidermis is required for fin regeneration

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Fig. 5

IGF signaling is required for the expression of blastemal and wound-epidermal marker genes. Whole-mount in situ hybridization of fins with antisense probes (purple). (A-D) In control fins at 36 hpa, fgf20a and msxB are strongly detected in the blastema of the outgrowth (A,C). In fins treated with NVP-AEW541, the expression of the blastemal marker genes is almost undetectable (B,D). (E-J). Drug-shift experiment (30 hours at normal conditions and 24 hours with treatment). Control fins express msxB in the blastema (E), wnt5b in the apical wound epidermis and distal tip of the blastema (G), and lef1 in the proximal wound epithelium and distal blastema (I). Exposure to NVP-AEW541 (F,H,J) strongly diminished expression of these genes. Scale bar: 100 μm.

Expression Data

Genes:	fgf20a lef1 msx1b wnt5b
Fish:	WT
Condition:	physical alteration: anatomical structure
Anatomical Terms:	apical epidermal cap basal regeneration epithelium blastema blastema distal side
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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