FIGURE

Fig. 4

ID

ZDB-FIG-100309-49

Publication

Poon et al., 2010 - Zebrafish cardiac enhancer trap lines: New tools for in vivo studies of cardiovascular development and disease

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Fig. 4

The CET line, ET31, marks a unique subset of myocardium at the early A-V valve. A: ET31 crossed with Tg(cmlc2:dsRed) showing a subset of myocardium positive for EGFP at the A-V junction and chambers (arrowhead). Note the EGFP expression at the BA. B, C: High magnification of A-V junction reveals differential GFP intensity between a different subset of myocardial cells. Arrows point to non-overlapped ET31 EGFP expression. D, E: High magnification of A-V junction lined with endocardial cells adjacent to cmlc2 marked myocardium (shown for comparison). Arrows point to A-V junction that ET31 marks. F-H: anti-gfp WISH demonstrates that the expression domain at the A-V node represents a ring. A-C: Double transgenic embryos of ET31 and Tg(cmlc2:dsRed). D, E, Double transgenic embryos of ET33-1A and Tg(cmlc2:dsRed). B, D: Fluorescent images. C, E: Composite fluorescent/DIC images to reveal cellular morphology. A, atrium; BA, bulbus arteriosus; A-V, atrio-ventricular valve; V, ventricle.

Expression Data

Gene:	EGFP
Fish:	sqet31Et
Anatomical Term:	atrioventricular valve
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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