FIGURE

Fig. 3

ID

ZDB-FIG-100302-40

Publication

Millimaki et al., 2010 - Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear

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Fig. 3

Relationship between Sox2 and upstream regulators of hair cell development. (A–C) Expression of brn3c:gfp in hs:atoh1a/+ transgenic embryos (A and C) and a hs:atoh1a/+;hs:sox2/+ double transgenic embryo (B) heat shocked at 24 hpf and photographed at 34 hpf. The specimen in (C) was also injected with sox2-MO. The inset in (A) shows a heat-shocked brn3c:gfp/+ control embryo at 34 hpf. (D–L) sox2 expression in a control embryo (D), atoh1a/b morphants (E and F), wild-type embryos exposed to SU5402 (G), DAPT (H), or both DAPT and SU5402 (I) beginning at 26 hpf, a hs:atoh1a/+ embryo heat shocked at 24 hpf (J), a hs:gal4/+;UAS-NICD/+ embryo heat shocked at 24 hpf (K), and a hs:fgf8/+ embryo heat shocked at 30 hpf (L). sox2 expression is shown at 30 hpf, except (D) and (E) (18 hpf) and (L) (36 hpf). Expression in control embryos does not change appreciably between 30 and 36 hpf. All images show lateral views with anterior to the left and dorsal to the top.

Expression Data

Genes:	GFP sox2
Fish:	WT x20Tg x17Tg s356tTg; x20Tg s356tTg; x20Tg; x21Tg kca3Tg/+; kca4Tg/+ s356tTg; x20Tg + MO2-sox2 WT + MO1-atoh1b + MO4-atoh1a
Conditions:	chemical treatment: DAPT chemical treatment: SU5402 chemical treatment: SU5402 heat shock
Knockdown Reagents:	MO1-atoh1b MO2-sox2 MO4-atoh1a
Anatomical Terms:	hair cell macula saccule macula utricle otic vesicle otic vesicle hair cell
Stage Range:	14-19 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s356tTg; x20Tg s356tTg; x20Tg; x21Tg s356tTg; x20Tg + MO2-sox2
Condition:	heat shock
Knockdown Reagent:	MO2-sox2
Observed In:	hair cell optic vesicle optic vesicle hair cell
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 338(2), Millimaki, B.B., Sweet, E.M., and Riley, B.B., Sox2 is required for maintenance and regeneration, but not initial development, of hair cells in the zebrafish inner ear, 262-269, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.