FIGURE

Fig. 5

ID

ZDB-FIG-091026-6

Publication

Hong et al., 2009 - FGF-dependent left-right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1

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Fig. 5

Cilia formation in Kupffer′s vesicle depends on Ier2 and Fibp1. (A–F) Monocilia were detected with anti-acetylated tubulin antibody (red), and nuclei with DAPI (blue) in Kupffer′s vesicle at the 5-somite stage. fgf8 MO, 8 ng (B) and ier2 MO plus fibp1 MO (E) -injected embryos show dramatic reduction of cilia, compared with control (A). Moderate reduction of cilia by ier2 MO (C) or fibp1 MO (D) alone was observed. Loss of cilia was rescued by coinjection with ier2 RNA plus fibp1 RNA with fgf8 MO (F). For levels of MOs see legend to Fig. 4. (G) Number of cilia in Kupffer′s vesicle; 12 embryos were counted in each case corresponding to images in (A–F). n, notochord.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-fgf8a AB + MO1-fibpb AB + MO1-ier2a AB + MO1-fibpb + MO1-ier2a
Knockdown Reagents:	MO1-fgf8a MO1-fibpb MO1-ier2a
Observed In:	Kupffer's vesicle cilium
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA