FIGURE

Fig. 6

ID

ZDB-FIG-091016-44

Publication

Norden et al., 2009 - Actomyosin is the main driver of interkinetic nuclear migration in the retina

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Actomyosin Is Active Basal to Apically Migrating Nuclei in the Retinal Neuroepithelium

(A) Confocal image three-dimensional reconstruction of GFP-Utr-CH labeling filamentous actin and H2B-RFP labeling nuclei. GFP-Utr-CH is seen at adhesion sites and plasma membranes. Arrows label prominent actin accumulation basal to nuclei.

(B) Images from Movie S14. Nuclei are labeled by H2B-RFP. After photoactivation of PaGFP-Utr-CH, it distributes more notably to the basal side of the plasma membrane beneath nucleus (arrowheads). Stable accumulations are seen basally of nucleus (arrow).

(C) Antibody staining with Phospho-Myosin Light Chain 2 antibodies for activated MyosinII (green) and plus DAPI (blue). Arrows mark basal accumulations of activated MyosinII.

(D) Images from Movie S15. Constitutively activated Myosin Light Chain is labeled by MRLC2^T18DS19D-GFP. Nuclei are labeled by H2B-RFP. Pink dot labels nucleus that migrates apically. Arrows label activated Myosin Light Chain dots that move apically basal of the nucleus.

Scale bars represent 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 138(6), Norden, C., Young, S., Link, B.A., and Harris, W.A., Actomyosin is the main driver of interkinetic nuclear migration in the retina, 1195-1208, Copyright (2009) with permission from Elsevier. Full text @ Cell