Fig. 6

nSR100 Regulates Vertebrate Nervous System and Sensory Organ Development

(A–C) RNA in situ hybridization assays monitoring ZnSR100 expression in the developing zebrafish embryo.

(A and A′) expression at 24 hpf, lateral and frontal views, respectively.

(B and B′) nSR100 expression at 36 hpf, lateral and dorsal views, respectively.

(C and C′) nSR100 expression at 48 hpf, lateral and dorsal views, respectively.

(D) p53 morpholino antisense oligonucleotide (MO) control-injected embryo at 50 hpf.

(E) p53 + ZnSR100 MOspl-injected embryo at 50 hpf.

(F) p53 + ZnSR100 MOspl-injected embryo at 50 hpf, rescued through coinjection of ZnSR100 mRNA.

(G–I) RNA in situ hybridization assays monitoring islet-1 expression in 50 hpf embryos. p53-MO-injected control (G), nSR100spl morphant embryo (H), and nSR100spl morphant embryo rescued through coinjection of 20 pg ZnSR100 mRNA (I) are shown.

(J–M) Confocal projections of trigeminal ganglion in Tg(sensory:GFP) transgenic embryos. Control embryo at 30 hpf (J), representative nSR100spl morphant embryo at 30 hpf (K), control embryo at 36 hpf (L), and representative nSR100spl morphant embryo at 36 hpf (M) are shown.

(N) nSR100-regulated alternative exons conserved between zebrafish and mouse display altered splicing patterns in nSR100spl morphant embryos, relative to control embryos. Total RNA was isolated from the heads of wild-type and nSR100spl morphant embryos at 50 hpf, and semiquantitative RT-PCR was performed to analyze AS patterns. Percent exon inclusion values are shown below each lane.