Fig. 6
Gli repressor activity is present in MZovl mutants. MZovl control zebrafish embryos (A,C,E,G) and morpholino-injected MZovl mutants (B,D,F,H) were stained at 24 hpf for the expression of ptc1 (A,B), eng2 (C-F) and nkx2.2a (G,H). (A-D) gli2aMO-treated MZovl embryos show enhanced ptc1 and eng2 expression as compared with uninjected MZovl controls. (E-H) gli3MO-treated MZovl embryos show enhanced eng2 expression and partially rescued nkx2.2a expression in the neural tube compared with uninjected MZovl controls. Arrowheads in G and H indicate the posterior extent of nkx2.2a expression in the neural tube. Lateral views. Scale bar: 100 μm. |