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Fig. S2

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ZDB-FIG-090716-16

Publication

Paridaen et al., 2009 - Apc1 is required for maintenance of local brain organizers and dorsal midbrain survival

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Fig. S2

Injection of Apc1, but not control MO, causes exacerbation of the apc mutant phenotype. All embryos at 48 hpf. Lateral view. Anterior to the left. In uninjected embryos, only wild-types (n = 38) and (‘mild’) apc (n = 18) phenotypes were observed. Injection of 0.25 mM apc1 MO into progeny of apc^+/- fish causes exacerbation of the apc phenotype (E–G), with 26% of injected embryos (total n = 61) showing no phenotype (E), 56% showing a ‘mild’ phenotype (F) and 18% showing a ‘severe’ phenotype (G). In contrast, injection of 0.25 mM of a 4 mismatch (4mm) control MO into progeny of apc^+/- fish did not affect the apc phenotype as only wild-type (C, n = 50) and ‘mild’ apc phenotypes (D, n = 10) were observed. In 4mm-injected wild-type and apc mutants, the size of the eye was somewhat reduced, indicating a non-specific effect of 4mm MO injection. Scale bar 250 μm.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 331(2), Paridaen, J.T., Danesin, C., Elas, A.T., van de Water, S., Houart, C., and Zivkovic, D., Apc1 is required for maintenance of local brain organizers and dorsal midbrain survival, 101-112, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.