FIGURE

Fig. 7

ID
ZDB-FIG-090710-57
Publication
Yabe et al., 2009 - The maternal-effect gene cellular island encodes aurora B kinase and is essential for furrow formation in the early zebrafish embryo
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Fig. 7

Cytokinesis defects in embryos lacking zygotic aurB function.

(A–C″) Wild-type (A), aurBhi1045 homozygote (B) and aurB morphant (C) embryos fixed at 24 hours p.f. and labeled to detect β-catenin (green) and DNA (red). (A–C) Overview of the head region. (A′–C′) Optical section through the brain region, showing internal cells. aurBhi1045 homozygotes and aurB morphants show a high frequency of compact nuclei which are typically arranged in pairs (some examples indicated with a white bar at their left flank), consistent with cell death after a failure to undergo proper cytokinesis. (A″–C″) Optical section through a surface layer of the same region, corresponding to the EVL or peridermal layer. In this layer, cells in aurBhi1045 homozygotes and aurB morphants show a high frequency of multilobular nuclei (some examples indicated by asterisks), again suggestive of defects in cytokinesis. Wild-type embryos injected with control MO exhibit normal cellular and nuclear morphologies (not shown), similar to those observed in untreated wild-type embryos (A). (D–E) High magnification images of a wild-type (D) and aurBhi1045 homozygous (E) embryos labeled to detect β-catenin (green), microtubules (red) and DNA (blue). Mutant embryos exhibit closely apposed pairs of nuclei (asterisks) that lack an intervening adhesive membrane.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Phenotype Detail
Acknowledgments
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