FIGURE

Fig. 2

ID

ZDB-FIG-090617-16

Publication

Park et al., 2009 - Isolation of a ventricle-specific promoter for the zebrafish ventricular myosin heavy chain (vmhc) gene and its regulation by GATA factors during embryonic heart development

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Fig. 2

Identical expression pattern of green fluorescent protein (GFP) reporter gene by vmhc promoter to endogenous vmhc mRNA in the heart region at the early heart-forming stage. A,C,E: Expression patterns of endogenous vmhc mRNA are shown in heart region at 18, 21.5, 24 hours postfertilization (hpf)each. Dorsal views with anterior to the left. B,D,F: Endogenous vmhc transcripts are observed in trunk muscle. Lateral view with anterior to the left. G,I,K: Expression patterns of gfp mRNA are observed in the heart region. Dorsal views with anterior to the left. H,J,L: No gfp transcript is observed in the trunk region. Lateral view with anterior to the left. M,N: GFP fluorescence signals from transgenic zebrafish embryos in heart region at 21.5 and 24 hpf. Dorsal views with anterior to the left. h, heart; tm, trunk muscle.

Expression Data

Gene:	EGFP
Fish:	knu9Tg (AB)
Anatomical Terms:	heart rudiment heart tube
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	knu9Tg + MO1-gata6 (AB) knu9Tg + MO5-gata4 (AB)
Knockdown Reagents:	MO1-gata6 MO5-gata4
Observed In:	heart morphogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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