Fig. 3

Heterozygous fish from high-tumor rp mutant lines are growth-impaired. A: Outcrosses of each rp line were performed, and their progeny were weighed and genotyped at day 25. White bars represent the average weight (mg) of heterozygotes (het) whereas gray bars represent the average weight (mg) of the wild-type (WT) siblings for each rp line; error bars are ±2 SEM. The P value (unpaired t-test) between heterozygotes and wild-type siblings is indicated above each set of bars. Lines are ordered along the x-axis by decreasing incidence of zMPNSTs. B: The growth defect determined in A, represented as the ratio of average heterozygote weight to average wild-type weight (grey bars), compared with the frequency of zMPNSTs (black bars), as determined in Table 1. The most highly tumor-prone lines appear to be the most severely growth-impaired. *indicates a statistically significant difference (P < 0.01 by unpaired t-test). Lines are ordered along the x-axis the same as in A for ease of comparison.