Fig. 5

Misregulation of pax2.1 and pax6 in nlz morphant fish in the setting of normal vax1 and vax2 expression at 24 hpf. In situ hybridization at 18 hpf (A–F) and 24 hpf (G–U). At 18 hpf, pax2.1 is normally expressed in the optic stalk and ventral optic primordium (A and D, arrowheads) and at 24 hpf is expressed distinctly in the optic stalk and fissure (G and J, arrowheads), midbrain/hindbrain boundary, and otic vesicle. In nlz1 morphant fish, pax2.1 expression is drastically reduced in the optic stalk and fissure (B, E, H, and K). In nlz2 morphant fish, the expression domain is expanded (C, F, I, L, open arrowheads). A higher magnification view shows the expression of pax2.1 in normal and nlz morphant fish at 18 hpf (D–F) and 24 hpf (J–L). (M–O) pax6 expression shows a subtle increase in the ventral eye in nlz1 morphant fish (N) and slight decrease in nlz2 fish (O, arrowheads). vax1 (P–R) and vax2 (S–U) are both expressed at normal levels and in a normal distribution in the optic stalk and ventral retina. (Scale bar, 100 μm.)