Fig. 2

(A) GFP expression patterns of different cis-element constructs during late blastula stage. The injected embryos were collected at 5 hpf for GFP visualization and photography. The embryos shown in lateral view with dorsal to the right. The A-Bp (A–C), B-Bp (D–F) and C-Bp (G–I) constructs show strong GFP expression in half of the embryo. The ABCR-Bp (J–K) construct shows strong GFP expression in the mesendoderm lineage. (A, D, G, J). The first column is a bright field image, the second column is green fluorescence and third column is a merged picture. (L) Endogenous sox17 expression pattern at 5 hpf. (B) Embryos injected with sox17 constructs have different GFP expression patterns during the gastrula stage. The injected embryos were collected at 8 hpf for GFP visualization and photography. The embryos were shown in lateral view with dorsal to the right. The A-Bp (A–C), B-Bp (D–F) and C-Bp (G–I) constructs show strong GFP expression in half of the embryo. The ABCR-Bp (J–K) construct shows strong GFP expression (A, D, G, J). The first column is a bright field image, the second column is green fluorescence and the third column is a merged picture. (L) Endogenous sox17 expression pattern at 8 hpf. (C) Double fluorescent in situ hybridization for egfp and endogenous genes The expression pattern for A-Bp-GFP (A–D), B-Bp-GFP (E–H), C-Bp-GFP (I–L), and ABCR-Bp-GFP (M–P) are obtained from embryos at 6 hpf. The egfp mRNA are indicated as green (C, G, K and O), foxa2 (B, F and J) and sox17 (N) mRNA are shown as red. The merged image (D, H, L and P) indicated the co-localization of egfp with endogenous foxa2 or sox17 at endoderm cells.