FIGURE

Fig. 7

ID

ZDB-FIG-090223-8

Publication

Vasilyev et al., 2009 - Collective Cell Migration Drives Morphogenesis of the Kidney Nephron

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Fig. 7

Decreasing Pronephric Fluid Flow Suppresses Anterior Migration

(A–D) Time-lapse frames of ET33-D10 GFP transgenics, 2.5 dpf. Time interval between frames is 2.5 h, scale bar: 80 μm (in bottom frame).

(A) polycystin2 morphant embryo; asterisk marks stationary cell in the pronephros.

(B) ift88 (polaris) morphant embryo; asterisks mark stationary cells in the pronephros.

(C) Anterior (proximal) obstruction; arrowhead marks position of unilateral anterior obstruction; white asterisk marks a migrating cell in the unobstructed nephron; black asterisk marks stationary cell in the obstructed nephron.

(D) Control morpholino injected embryo showing normal rate of proximal cell migration (asterisk marks a migrating cell).

(E) Measurement of proximal nephron segment shortening in ET33-D10 GFP transgenics (shorter lengths indicate further proximal progression as described in Figure 5E). From left to right: Control uninjected embryos (n = 36); polycystin2 morphant embryos (n = 20); ift88 (polaris) morphant embryos (n = 10); Control morpholino injected embryos (n = 17)

(F) Rates of proximal cell migration, from left to right: Control; polycystin2 morphant embryo; ift88 (polaris) morphant embryo; anterior obstruction; Control morpholino.

Expression Data

Gene:	EGFP
Fish:	sqet33D10Et + MO2-ift88 (AB) sqet33D10Et + MO7-pkd2 (AB)
Knockdown Reagents:	MO2-ift88 MO7-pkd2
Anatomical Term:	pronephros cell
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sqet33D10Et + MO2-ift88 (AB) sqet33D10Et + MO7-pkd2 (AB)
Knockdown Reagents:	MO2-ift88 MO7-pkd2
Observed In:	cell migration cell motility proximal convoluted tubule development
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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