FIGURE

Fig. 6

ID

ZDB-FIG-090127-37

Publication

Sittaramane et al., 2009 - The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish

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Fig. 6

Genetic interactions between stbm and lama1. All panels show dorsal views of the hindbrain with anterior to the left. Tg(isl1:gfp) embryos were fixed at 48 hpf, and processed for immunohistochemistry with zn5 antibody (red) to label dorsal commissural neurons and axons at rhombomere boundaries (A–F), and anti-GFP antibody (green) to label FBMNs (A–H; arrowheads). (A) FBMNs migrate normally in a control embryo. (B, C) Partial loss of FBMN migration in embryos injected with suboptimal dose of lama1 MO (B) or stbm MO (C). (D) Complete loss of FBMN migration in an embryo injected with suboptimal doses of lama1 and stbm MOs. (E) Partial loss of FBMN migration in a trilobite^tc240a (tri) heterozygous (stbm +/-) embryo. (F) Complete loss of FBMN migration in a trilobite heterozygote injected with suboptimal dose of lama1 MO. (G) Greatly reduced FBMN migration in a bashful^b765 homozygous (lama1 -/-) embryo. (H) Complete loss of FBMN migration in a trilobite; bashful double heterozygote. Scale bar in H (75 μm for A–H).

Expression Data

Gene:	GFP
Antibody:	zn-5
Fish:	vangl2^tc240/+ rw0Tg rw0Tg + MO1-lama1 rw0Tg + MO1-vangl2 lama1^b765/b765; rw0Tg vangl2^tc240/+; rw0Tg rw0Tg + MO1-lama1 + MO1-vangl2 vangl2^tc240/+; lama1^b765/+; rw0Tg vangl2^tc240/+; rw0Tg + MO1-lama1
Knockdown Reagents:	MO1-lama1 MO1-vangl2
Anatomical Terms:	brainstem and spinal white matter facial nerve motor nucleus motor neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg + MO1-lama1 rw0Tg + MO1-vangl2 lama1^b765/b765; rw0Tg vangl2^tc240/+; rw0Tg rw0Tg + MO1-lama1 + MO1-vangl2 vangl2^tc240/+; lama1^b765/+; rw0Tg vangl2^tc240/+; rw0Tg + MO1-lama1
Knockdown Reagents:	MO1-lama1 MO1-vangl2
Observed In:	facial nerve motor nucleus motor neuron neuron migration
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 325(2), Sittaramane, V., Sawant, A., Wolman, M.A., Maves, L., Halloran, M.C., and Chandrasekhar, A., The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish, 363-373, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.