FIGURE

Fig. 2

ID

ZDB-FIG-081222-2

Publication

Montpetit et al., 2008 - Disruption of AP1S1, causing a novel neurocutaneous syndrome, perturbs development of the skin and spinal cord

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Morphological phenotype of Ap1s1 knockdown zebrafish is rescued by over expression of human AP1S1.

Transmitted light images of 48 hpf Ap1s1 KD larvae show their smaller size, reduced pigmentation (D) and skin disorganization (E) compared to the WT (A, B) and the rescued larvae (G,H). Immunofluorescence of wholemount zebrafish using anti-Ap1s1 antibody showing localization of Ap1s1 to the plasma membrane (C, polygonal) and to a well defined perinuclear ring, in both normal (C) and rescued larvae (G), whereas only a residual and diffuse staining could be observed in KD larvae (F). Western blot analysis (D, inset) indicates nearly complete knockdown of Ap1s1 protein (WT = wild-type, KD = knockdown, CTRL = control rat brain proteins). To normalize the western blot analysis, proteins extracted from WT, KD and CTRL larvae were incubated with anti-actin. Scale bars in (A, D, G) = 100 μm, (B, C, E, F, H, I-Ciii) = 50 μm.

Expression Data

Gene:	ap1s1
Fish:	WT
Anatomical Terms:	caudal fin nuclear outer membrane caudal fin plasma membrane whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ap1s1
Knockdown Reagent:	MO1-ap1s1
Observed In:	caudal fin integument pigmentation whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.