Fig. S5

Assessment of proliferation, cell death, and differentiation in bbs8 morphant NC. (A and B) Expression of Sox10:eGFP in 72 hpf zebrafish shows a lack of pharyngeal arches in morphants relative to controls (arrowheads in B). Also, the number of proliferating Sox10:eGFP-positive cells shown by Ph3 staining (in red) is not different between control and morphant. (C and D) Sox10:eGFP expression in the head of 24 hpf fish showing the lack of migration in bbs8 morphants. Ph3 staining again shows no difference in the amount of proliferation in the crest cells despite less proliferation in cells of the brain. (E and F) Sox10:eGFP cells migrating into the trunk at 30 hpf do not inappropriately differentiate into neurons in the bbs8 morphants as revealed by HuC/D immunostaining (in red). (G and H) FACS plots (G) of embryos show that control and morphant Sox10:eGFP embryos have similar proportions of neural crest cells (H), indicating that proliferation of the NCCs is not affected in the morphants.