FIGURE

Fig. 6

ID

ZDB-FIG-080828-87

Publication

Kim et al., 2008 - Notch-regulated oligodendrocyte specification from radial glia in the spinal cord of zebrafish embryos

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Fig. 6

Notch signaling is required to maintain radial glial cells during embryonic development. All panels show transverse sections of spinal cord with dorsal upward. A-C: Tg(olig2:egfp) larvae labeled with anti-Zrf-1 antibody (red) at 6 days postfertilization (dpf). Control (A), DAPT treatment begun at 32 hours postfertilization (hpf). (B), and DAPT treatment begun at 50 hpf (C). Arrows indicate Zrf-1⁺ enhanced green fluorescent protein-positive (EGFP⁺) radial glial cells, and arrowheads indicate EGFP⁺ oligodendrocyte lineage cells. D-F: A 3 dpf Tg(hsp70l:GAL4);Tg(UAS:myc-Notch1a-intra) larvae labeled with Zrf-1 antibody; control (D), heat induction at 24 hpf (E), and heat induction at 50 hpf (F). G,H: A 3 dpf larvae probed to detect her4 expression by in situ RNA hybridization. Control (G) and DAPT treatment begun at approximately 50 hpf (H). Scale bar = 20 μM.

Expression Data

Genes:	EGFP her4.1
Antibody:	zrf-1
Fish:	AB vu12Tg kca3Tg; kca4Tg
Conditions:	chemical treatment: DAPT heat shock
Anatomical Terms:	glioblast (sensu Vertebrata) radial glial cell spinal cord
Stage Range:	Protruding-mouth to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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