FIGURE

Fig. S3

ID

ZDB-FIG-080715-32

Publication

Liu et al., 2008 - In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal

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Fig. S3

Hypomorphic fgf3 knock down do not inhibit POMC-GFP cell proliferation. Developmental stages are indicated at bottom right corner of each panel. (A-C) standard control MO injection. (D-F) fgf3 MO injection. DIC images of pituitary ventral-lateral views superimposed with fluorescent images of POMC-GFP expression (A and D), or BrdU immunostain in red (B and E). Fluorescent images of POMC-GFP superimposed with BrdU labeled cell in red show no inhibition of POMC-GFP cell proliferation in hypomorphic FGF3 morphants (F) compared with embryos injected with standard control MO (C) . Scale bar, 50 μm. All ventral-lateral view; top, anterior; right, dorsal; arrowhead: anterior-most POMC cells; arrow: lateral-posterior POMC cells.

Expression Data

Gene:	GFP
Fish:	zf44Tg zf44Tg + MO1-fgf3
Knockdown Reagent:	MO1-fgf3
Anatomical Term:	adenohypophyseal placode
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 319(2), Liu, N.A., Ren, M., Song, J., Ríos, Y., Wawrowsky, K., Ben-Shlomo, A., Lin, S., and Melmed, S., In vivo time-lapse imaging delineates the zebrafish pituitary proopiomelanocortin lineage boundary regulated by FGF3 signal, 192-200, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.