FIGURE

Fig. 5

ID

ZDB-FIG-080617-10

Publication

Anderson et al., 2008 - Loss of unc45a precipitates arteriovenous shunting in the aortic arches

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Fig. 5

Lack of blood flow can rescue AVM formation in kus^tr12 mutants. Ninety-eight percent (n = 117) of wild type or heterozygous embryos treated with 2,3-butanedione monoxime (BDM; stops heart beat) between 47 and 53 hpf exhibited normal aortic arches (A), ventral aorta (C), and jaw and pharyngeal cartilages (E). This treatment rescued aortic arch (B) and ventral aorta (D) phenotypes in 67% of kus^tr12 mutants (n = 29), but had no effect on the basibranchial cartilage phenotype (vertical lines in Panel F). A–D: 2D confocal projections of 74 hpf TG(flk1:GFP^la116;gata1:dsRed) embryos, labeling endothelial and blood cells green and red, respectively. G–H: Macro images of 4 dpf embryos stained with anti-collagen II. A, B: lateral views, anterior the left. C–F: ventral views, anterior to the left. PHS, primary head sinus. VA, ventral aorta.

Expression Data

Genes:	DsRed GFP
Antibody:	Ab1-col2a
Fish:	WT unc45a^tr12/tr12 la116Tg; sd2Tg unc45a^tr12/tr12; la116Tg; sd2Tg
Condition:	chemical treatment: diacetylmonoxime
Anatomical Terms:	aortic arch blood cell blood vessel endothelial cell pharyngeal arch cartilage primary head sinus ventral aorta
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	unc45a^tr12/tr12 unc45a^tr12/tr12; la116Tg; sd2Tg
Condition:	chemical treatment: diacetylmonoxime
Observed In:	aortic arch ceratobranchial cartilage ventral aorta
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 318(2), Anderson, M.J., Pham, V.N., Vogel, A.M., Weinstein, B.M., and Roman, B.L., Loss of unc45a precipitates arteriovenous shunting in the aortic arches, 258-267, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.