FIGURE

Fig. 3

ID

ZDB-FIG-080502-21

Publication

Farooq et al., 2008 - Histone deacetylase 3 (hdac3) is specifically required for liver development in zebrafish

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Fig. 3

Liver formation in zebrafish does not require vascularization. Zebrafish embryos from triple transgenic line were treated with VPA (20 μM). Severe defects in intersomatic blood vessels (ISV) (thin white arrows) and subintestinal blood vessels (SIV) (wide white arrow) were observed up to 5 dpf (E–H). Liver only appeared on 5 dpf and no or minimum liver vascularization was observed (H). Similar angiogenesis defects were observed in embryos treated with angiogenesis inhibitors SU5614 (5 μM, I–L) and SU1498 (5 μM, M–P). However, liver (thick white arrows) formed normally on 3 dpf and grew extensively from 3 dpf to 5dpf in these embryos (I–P). All images are lateral view, anterior toward left. The images D, H, L and P are confocal images of merged z-stacks. The scale bar is 100 μm in panel A, and 30 μm in panel D.

Expression Data

Genes:	DsRed EGFP
Fish:	gz15Tg; y1Tg
Conditions:	chemical treatment: SU1498 chemical treatment: SU5614 chemical treatment: sodium valproate
Anatomical Terms:	blood vasculature liver
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 317(1), Farooq, M., Sulochana, K.N., Pan, X., To, J., Sheng, D., Gong, Z., and Ge, R., Histone deacetylase 3 (hdac3) is specifically required for liver development in zebrafish, 336-353, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.