FIGURE

Fig. 4

ID

ZDB-FIG-080424-4

Publication

Wallace et al., 2003 - Unique and conserved aspects of gut development in zebrafish

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Fig. 4

The zebrafish liver and pancreas form independently of the gut tube. (A, B) Whole-mount RNA in situ hybridization using a ceruloplasmin (cer) probe, dorsal view. Compared with WT (A), liver duplication (arrows) is seen in 50-hpf ethanol-treated embryos (B). (C, D) Histological cross-sections through the liver of 50-hpf WT (C) and ethanol-treated (D) embryos processed for cer in situs. Anterior endoderm spans the midline between the duplicated livers (arrows in D) of ethanol-treated embryos. (E, F) Laminin immunostainings from a comparable region of 50-hpf WT (E) and ethanol-treated (F) embryos. Organized endoderm present within the developing esophagus (white arrow) of 50-hpf WT embryos is not identifiable in ethanol-treated embryos (F). (G) Histological cross-sections through the rostral gut of 50-hpf WT embryo. (H) Endoderm in a comparable region of 50-hpf ethanol-treated embryo spans the midline and is unorganized. (I, J) Immunoreactive laminin in a WT 50-hpf embyro (I) encircles the gut tube, but is widely dispersed in unorganized endoderm of ethanol-treated embryos (J). Dotted line in (A, B) represents the embryonic midline. Circle in (G) surrounds the gut tube.

Expression Data

Gene:	cp
Antibody:	Ab1-lama1
Fish:	WT
Condition:	chemical treatment: ethanol
Anatomical Terms:	endoderm gut liver
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment: ethanol
Observed In:	esophagus intestinal rod liver
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 255(1), Wallace, K.N. and Pack, M., Unique and conserved aspects of gut development in zebrafish, 12-29, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.