FIGURE

Fig. S4

ID

ZDB-FIG-080422-15

Publication

Chung et al., 2008 - Intra-endodermal interactions are required for pancreatic beta cell induction

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Fig. S4

Smoothened Function in Adaxial and Lateral Floor Plate Cells Does Not Appear to Be Required for the Induction of Dorsal Pancreatic β-Cells
(A) Schematic diagram of the cell transplantation protocol. Wildtype Tg(ins:GFP) donor cells were transplanted into wildtype or smo mutant Tg(ins:GFP) hosts. (B-G) Transverse confocal images of wildtype (B and E) and smo mutant (C, D, F and G) hosts stained for GFP (green) and β-catenin (white) at 18 hpf. Wildtype donor cells are labeled with rhodamine dextran (red). (B and E) Regardless of the position of the wildtype donor cells, Tg(ins:GFP) expression is unaffected in wildtype hosts. (C, D, F and G) 13 Tg(ins:GFP) expression is not rescued in smo mutant hosts when wildtype donor cells differentiate into adaxial (C and F; arrowhead) or lateral floor plate (D and G; arrowhead) cells in the pancreatic region, i.e. between the 1^st and 4^th somites.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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Reprinted from Developmental Cell, 14(4), Chung, W.S., and Stainier, D.Y., Intra-endodermal interactions are required for pancreatic beta cell induction, 582-593, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell